ythdf3 proteintech 25537 1 ap Search Results


rabbit ythdf3  (Proteintech)
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Proteintech rabbit ythdf3
G3BP1 interactome differs in MNV-infected cells and arsenite treated cells as addressed by immunofluorescence (A-C). BV2 GFP-G3BP1 cells were either infected with MNV (MOI 10) for 9h p.i. or treated with arsenite 0.1mM for 1h and subcellular colocalisation of candidate proteins identified by mass spectrometry as interacting with GFP-G3BP1 was addressed by immunostaining (magenta) in GFP-G3BP1 positive cells (cyan). MNV-infected cells were detected by immunostaining against dsRNA (gold), nuclei were stained with DAPI. Scale bars, 10μm, red arrows indicate example of colocalisation between GFP-G3BP1 and a candidate protein. Representative view (n = 3) of confocal analysis using staining against (A) <t>YTHDF3,</t> candidate for arsenite-induced SG only, (B) FMR1, candidate for both arsenite- and MNV-induced G3BP1 granules, (C) Rab7, candidate for MNV-induced G3BP1 granules only.
Rabbit Ythdf3, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ythdf3+proteintech+25537+1+ap/YTHDF3-specific+Antibody/pmc06964919-271-23-26
Average 96 stars, based on 1 article reviews
rabbit ythdf3 - by Bioz Stars, 2026-10
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G3BP1 interactome differs in MNV-infected cells and arsenite treated cells as addressed by immunofluorescence (A-C). BV2 GFP-G3BP1 cells were either infected with MNV (MOI 10) for 9h p.i. or treated with arsenite 0.1mM for 1h and subcellular colocalisation of candidate proteins identified by mass spectrometry as interacting with GFP-G3BP1 was addressed by immunostaining (magenta) in GFP-G3BP1 positive cells (cyan). MNV-infected cells were detected by immunostaining against dsRNA (gold), nuclei were stained with DAPI. Scale bars, 10μm, red arrows indicate example of colocalisation between GFP-G3BP1 and a candidate protein. Representative view (n = 3) of confocal analysis using staining against (A) YTHDF3, candidate for arsenite-induced SG only, (B) FMR1, candidate for both arsenite- and MNV-induced G3BP1 granules, (C) Rab7, candidate for MNV-induced G3BP1 granules only.

Journal: PLoS Pathogens

Article Title: Norovirus infection results in eIF2α independent host translation shut-off and remodels the G3BP1 interactome evading stress granule formation

doi: 10.1371/journal.ppat.1008250

Figure Lengend Snippet: G3BP1 interactome differs in MNV-infected cells and arsenite treated cells as addressed by immunofluorescence (A-C). BV2 GFP-G3BP1 cells were either infected with MNV (MOI 10) for 9h p.i. or treated with arsenite 0.1mM for 1h and subcellular colocalisation of candidate proteins identified by mass spectrometry as interacting with GFP-G3BP1 was addressed by immunostaining (magenta) in GFP-G3BP1 positive cells (cyan). MNV-infected cells were detected by immunostaining against dsRNA (gold), nuclei were stained with DAPI. Scale bars, 10μm, red arrows indicate example of colocalisation between GFP-G3BP1 and a candidate protein. Representative view (n = 3) of confocal analysis using staining against (A) YTHDF3, candidate for arsenite-induced SG only, (B) FMR1, candidate for both arsenite- and MNV-induced G3BP1 granules, (C) Rab7, candidate for MNV-induced G3BP1 granules only.

Article Snippet: Primary antibodies dilutions: rabbit anti-NS3 (1:600), mouse anti-dsRNA (1:1,000), mouse anti-puromycin (1:5, http://dshb.biology.uiowa.edu/PMY-2A4 ), mouse anti-G3BP1 (1:400, Invitrogen), goat anti-eIF3B (1:400, Santa Cruz), rabbit YTHDF3 (1:600, Proteintech), rabbit anti-RAB7A (1:600, Proteintech), rabbit anti-FMR1 (1:600, Novusbio).

Techniques: Infection, Immunofluorescence, Mass Spectrometry, Immunostaining, Staining